Creatine Kinase MB (CK-MB) quantitative detection kit (colloidal gold method) uses the principle of double antibody sandwich method and immunochromatography gold immnnochromatography technique to quickly detect CK-MB in whole blood of human beings, coat gold immnnochromatography CK-MB monoclonal antibody and mouse monoclonal antibody on polyester fiber (or non-wovens) and respectively coat CK-MB monoclonal antibody and anti-mouse IgG antibody in detection line and quality control line of nitrocellulose membrane. During measurement, drop the serum samples into test paper sampling area and the mixture are chromatographed upwardly from the bottom under capillary effect. If CK-MB concentration in the sample is higher than 2ng/ml, the colloidal gold will first combine with CK-MB antigen in the sample in the chromatographic process to form a compound, and then the compound will move forward along the paper slip under chromatography effect and form “Au-CK-MB Ab-CK-MB Ag- CK-MB Ab” sandwiched object in combination with the procated CK-MB monoclonal antibodies when passing the detection line, thus displaying amaranthine stripe at the detection line. As CK-MB concentration in the sample increases, the color development at the detection line becomes deepened. The free gold immnnochromatography mouse monoclonal antibody is combined with anti-mouse IgG antibody at the quality control line, thus displaying amaranthine stripe at the quality control line to determine whether the amount of sampling added is accurate and whether the chromatography is consistent with the normal standard.
The product calibration card of the corresponding batch includes the standard curve information of the same batch of products and is detected by detector after the sample is added; the signal is obtained by collecting the grey value of the detection line, and the instrument will automatically make comparative analysis to grey value and standard curve to obtain CK-MB content in the sample.
Model & Spec.
CK-MB test paper strip
It is composed of test paper strip, plastic card, drying agent and aluminum foil bag while test paper strip is constituted by plastic substrate, colloidal gold pad, cellulose membrane, sampling adding pad and absorption pad. Colloidal gold pad is pre-peridium with gold labeling CK-MB monoclonal antibody and rat monoclonal antibody, nitrocellulose membrane detection line and quality control line are peridium separately with CK-MB monoclonal antibody and anti-rat IgG antibody.