Cardiac Troponin I(cTnI) quantitative detection kit (colloidal gold method) uses the principle of double antibody sandwich method and immunochromatography gold immnnochromatography technique to quickly detect Cardiac Troponin I (cTnI) in whole blood of human beings, coat gold immnnochromatography cTnI monoclonal antibody and mouse monoclonal antibody on polyester fiber (or non-wovens) and respectively coat cTnI monoclonal antibody and mouse monoclonal antibody in detection line and quality control line of nitrocellulose membrane. During measurement, drop the whole blood specimen into test paper sampling area, and the mixture is chromatographed upwardly from the bottom under capillary effect. If cTnI concentration in the sample is higher than 0.5ng/ml, the colloidal gold will first combine with cTnI antigen in the sample in the chromatographic process to form a compound, and then the compound will move forward along the paper slip under chromatography effect and form “Au-cTnI Ab-cTnI Ag-cTnI Ab” sandwiched object in combination with the pre-coat cTnI monoclonal antibodies when passing the detection line, thus displaying amaranthine stripe at the detection line. As cTnI concentration in the sample increases, the color development at the detection line becomes deepened. The free gold immnnochromatography mouse monoclonal antibody is combined with mouse IgG antibody at the quality control line, thus displaying amaranthine stripe at the quality control line to determine whether the amount of sampling added is accurate and whether the chromatography is consistent with the normal standard.
The product calibration card of the corresponding batch includes the standard curve information of the same batch of products and is detected by detector after the sample is added; the signal is obtained by collecting the grey value of the detection line, and the instrument will automatically make comparative analysis to grey value and standard curve to obtain cTnI content in the sample.
Model & Spec.
cTnI test paper strip
It is composed of test paper strip, plastic card, drying agent and aluminum foil bag while test paper strip is constituted by plastic substrate, colloidal gold pad, cellulose membrane, sampling adding pad and absorption pad. Colloidal gold pad is pre-peridium with gold labeling cTnI monoclonal antibody and rat monoclonal antibody, nitrocellulose membrane detection line and quality control line are peridium separately with cTnI monoclonal antibody and anti-rat IgG antibody.