HBcAb ELISA test kit

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Overview
Quick Details
Type:
Pathological Analysis Equipments
Brand Name:
EGENS
Place of Origin:
Jiangsu, China
Certificate:
ISO9001,ISO13485
Storage:
2-30℃
Shelf Time:
2 years
Packaging & Delivery
Packaging Details
96T/48T per box
Port
Shanghai port
Lead Time :
after payment within 30days

Specifications

rapid test kits
1.easy to use
2.easy to read
3.High accuracy
4.High speed

INSTRUCTIONS FOR USE
HBcAb ELISA
For the detection of antibody to Hepatitis B C Antigen
in human serum or plasma (in vitro)

INTENDED USE
This HBcAb ELISA is a qualitative enzyme immunoassay for the in vitro detection of antibody to Hepatitis B C
Antigen (HBcAg) in human serum or plasma. It is intended to use in medical laboratories for diagnosis and
management of patients related to infection with hepatitis B virus.
COMPONENTS
1. Microplate 96 wells
2. Negative Control 1 mL ×1
3. Positive Control 1 mL ×1
4. Conjugate 6 mL ×1
5. Washing Buffer (20×) 20 mL ×1
6. Substrate Solution A 6 mL ×1
7. Substrate Solution B 6 mL ×1
8. Stop Solution 6 mL ×1
9. Plate Cover 2 pieces
10. Insert 1 copy

 

ASSAY PROCEDURE
1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer (20×)with 19 volumes of distilled water,
mix well.
2. Add Samples and Conjugate: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells
as negative control, 2 wells as positive control. After dispensing 50µL Samples and 50µL Negative Control or
Positive Control to the respective wells, add 50μl of Conjugate into each well except the blank. Gently
vibrating the plate.
3. Incubate: Cover the Microplate with Plate Cover and incubate the Microplate in a thermostat-controlled
water-bath or microplate incubator at 37℃ for 30 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted
washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure
that the rest volume is minimal, by tapping plate onto absorbent paper.
5. Add Substrate: Add 50µL of Substrate Solution A and 50µL of Substrate Solution B to each well, mix well.
Cover and incubate at 37℃ for 10 minutes.
6. Stop reaction: Add 50µL Stop Solution to each well, mix well.
7. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should
be selected from 620nm to 690nm.

 

RESULTS
1. For the assay to be valid, the mean OD value of positive controls must be less than or equal to 0.1 and the
mean OD value of negative controls must be greater than or equal to 0.8.
2. Cut off value = PC×0.6+NC×0.4
PC= the mean absorbance value for two positive controls
NC= the mean absorbance value for two negative controls
Important: If the NC is higher than 1.5, take it as 1.5.
3. OD value of the sample ≥ cut off value, it is negative for HBcAb;
OD value of the sample < cut off value, it is positive for HBcAb.
PRECAUTIONS
1. Allow all kit components to reach room temperature before use.
2. Follow the direction insert to control the reaction temperature and time strictly.
3. Do not mix components of different lot numbers to use.
4. The kit should be store at 2-8℃. Do not use kit components beyond their expiration date.

 

 

 

 

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