1.easy to use
2.easy to read
INTENDED USE This HBsAb ELISA Quantitatve is a an enzyme linked immunosorbent assay for in vitro quantitative detection of antibodies to hepatitis B virus surface antigen (anti-HBs) in human serum or plasma for clinical purposes and assessing antibody response levels to HBsAg-vaccine. COMPONENTS 1. Microplate 96 wells 2. Calibration Curve Standards 0.5 mL×6 3. Conjugate 6 mL ×1 4. Washing Buffer (20×) 20 mL ×1 5. Substrate Solution A 6 mL ×1 6. Substrate Solution B 6 mL ×1 7. Stop Solution 6 mL ×1 8. Plate Cover 2 pieces 9. Insert 1 copy
1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer (20×)with 19 volumes of distilled water, mix well. 2. Add Samples and Conjugate: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 12 wells as Calibration Curve Standards (run the standards in duplicates), 0 mIU/ml, 10 mIU/ml, 20 mIU/ml, 40 mIU/ml, 80 mIU/ml, 160 mIU/ml. After dispensing 50µL Samples and 50µL Calibration Curve Standards to the respective wells, add 50μl of Conjugate into each well except the blank. Gently vibrating the plate. 3. Incubate: Cover the Microplate with Plate Cover and incubate the Microplate in a thermostat-controlled water-bath or microplate incubator at 37°C for 60 minutes. 4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper. 5. Add Substrate: Add 50µL of Substrate Solution A and 50µL of Substrate Solution B to each well, mix well. Cover and incubate at 37°C for 10 minutes. 6. Stop reaction: Add 50µL Stop Solution to each well, mix well. 7. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.