HBsAb ELISA Kit (Elisa kit)

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Overview
Quick Details
Type:
Pathological Analysis Equipments
Brand Name:
EGENS
Place of Origin:
Jiangsu, China (Mainland)
Certificate:
ISO9001,ISO13485
Storage:
2-30℃
Shelf Time:
2 years
Packaging & Delivery
Packaging Details
96T/KIT
Port
Shanghai port
Lead Time :
after payment within 30days

Specifications

elisa kit
1.easy to use
2.easy to read
3.High accuracy
4.High speed

elisa kit

Product Description

 

  

INTENDED USE
This HBsAb ELISA Quantitatve is a an enzyme linked immunosorbent assay for in vitro quantitative detection of
antibodies to hepatitis B virus surface antigen (anti-HBs) in human serum or plasma for clinical purposes and
assessing antibody response levels to HBsAg-vaccine.
COMPONENTS              
1. Microplate           96 wells
2. Calibration Curve Standards                                0.5 mL×6
3. Conjugate           6 mL ×1
4. Washing Buffer (20×)        20 mL ×1
5. Substrate Solution A         6 mL ×1
6. Substrate Solution B         6 mL ×1
7. Stop Solution          6 mL ×1
8. Plate Cover          2 pieces
9. Insert            1 copy 


ASSAY PROCEDURE

1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer  (20×)with 19 volumes of distilled water,
mix well.
2. Add Samples and Conjugate: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 12 wells
as Calibration Curve Standards (run the standards in duplicates), 0 mIU/ml, 10 mIU/ml, 20 mIU/ml, 40
mIU/ml, 80 mIU/ml, 160 mIU/ml. After dispensing 50µL Samples and 50µL Calibration Curve Standards to
the respective wells, add 50μl of Conjugate into each well except the blank. Gently vibrating the plate. 
3. Incubate: Cover the Microplate with Plate Cover  and incubate the Microplate in a thermostat-controlled
water-bath or microplate incubator at 37°C for 60 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted
washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure
that the rest volume is minimal, by tapping plate onto absorbent paper.
5. Add Substrate: Add 50µL of Substrate Solution A and 50µL of Substrate Solution B to each well, mix well.
Cover and incubate at 37°C for 10 minutes.
6. Stop reaction: Add 50µL Stop Solution to each well, mix well.
7. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should
be selected from 620nm to 690nm.