1.easy to use
2.easy to read
INTENDED USE This HBeAg ELISA is a qualitative enzyme immunoassay for the in vitro detection of Hepatitis B E Antigen (HBeAg) in human serum or plasma. It is intended to use in medical laboratories for diagnosis and management of patients related to infection with hepatitis B virus. COMPONENTS 1. Microplate 96 wells 2. Negative Control 1 mL ×1 3. Positive Control 1 mL ×1 4. Conjugate 6 mL ×1 5. Washing Buffer (20×) 20 mL ×1 6. Substrate Solution A 6 mL ×1 7. Substrate Solution B 6 mL ×1 8. Stop Solution 6 mL ×1 9. Plate Cover 2 pieces 10. Insert 1 copy
ASSAY PROCEDURE 1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer (20×)with 19 volumes of distilled water, mix well. 2. Add Samples and Conjugate: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 50µL Samples and 50µL Negative Control or Positive Control to the respective wells, add 50μl of Conjugate into each well except the blank. Gently vibrating the plate. 3. Incubate: Cover the Microplate with Plate Cover and incubate the Microplate in a thermostat-controlled water-bath or microplate incubator at 37°C for 30 minutes. 4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper. 5. Add Substrate: Add 50µL of Substrate Solution A and 50µL of Substrate Solution B to each well, mix well. Cover and incubate at 37°C for 10 minutes. 6. Stop reaction: Add 50µL Stop Solution to each well, mix well. 7. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.