HBsAg ELISA Kit (Elisa kit)

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Overview
Quick Details
Type:
Pathological Analysis Equipments
Brand Name:
EGENS
Place of Origin:
Jiangsu, China
Certificate:
ISO9001,ISO13485
Storage:
2-30℃
Shelf Time:
2 years
Packaging & Delivery
Packaging Details
96T/KIT
Port
Shanghai port
Lead Time :
after payment within 30days

Specifications

Elisa kit
1.easy to use
2.easy to read
3.High accuracy
4.High speed

HBsAg ELISA Kit (Elisa kit)

 

INTENDED USE 

This HBsAg ELISA is a qualitative enzyme immunoassay for the in vitro detection of Hepatitis B Surface
Antigen(HBsAg) in human serum or plasma. It is intended for screening of blood donors and diagnosing patients
related to infection with HBsAg.
COMPONENTS              
1. Microplate           96 wells
2. Negative Control         1 mL ×1
3. Positive Control         1 mL ×1
4. Conjugate           6 mL ×1
5. Washing Buffer (20×)        20 mL ×1
6. Substrate Solution A         6 mL ×1
7. Substrate Solution B         6 mL ×1
8. Stop Solution          6 mL ×1
9. Plate Cover          2 pieces
10. Insert            1 copy 

ASSAY PROCEDURE
1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer  (20×)with 19 volumes of distilled water,
mix well.
2. Add Samples and Conjugate: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells
as negative control, 2 wells as positive control. After dispensing 50µL Samples and 50µL Negative Control or
Positive Control to the respective wells, add 50μl of Conjugate into each well except the blank. Gently
vibrating the plate. 
3. Incubate: Cover the Microplate with Plate Cover  and incubate the Microplate in a thermostat-controlled
water-bath or microplate incubator at 37°C for 30 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted
washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure
that the rest volume is minimal, by tapping plate onto absorbent paper.
5. Add Substrate: Add 50µL of Substrate Solution A and 50µL of Substrate Solution B to each well, mix well.
Cover and incubate at 37°C for 10 minutes.
6. Stop reaction: Add 50µL Stop Solution to each well, mix well.
7. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should
be selected from 620nm to 690nm.

 

 

 

  

 

 

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